BIOLOGIC
INSIGHTS
⚡ NEET 2025 Quizz ⚡
Genetic Engineering - NEET PYQs (2004-2024)
Master Recombinant DNA Technology with 50+ NEET Questions
NEET Genetic Engineering - Must-Know Concepts
🔧 Tools of rDNA Technology
- Restriction Enzymes: Molecular scissors (EcoRI, HindIII)
- DNA Ligase: Joins DNA fragments
- Vectors: pBR322, Ti plasmid, BAC, YAC
- Host Cells: E. coli, yeast, mammalian cells
⚙️ Processes in Genetic Engineering
- Gene Cloning Steps: Isolation → Cutting → Ligation → Transformation → Screening
- PCR: Denaturation → Annealing → Extension (Taq polymerase)
- Blotting Techniques: Southern (DNA), Northern (RNA), Western (protein)
💡 Applications
- Medicine: Insulin, HGH, vaccines (Hepatitis B)
- Agriculture: Bt crops, Golden rice
- Forensics: DNA fingerprinting (VNTRs)
- Gene Therapy: ADA-SCID treatment
NEET Trend Analysis (Last 10 Years):
- Restriction enzymes & vectors: 3-5 questions/year
- PCR applications: 100% appearance rate
- Biotech products: Insulin, Bt crops frequently asked
🚨 NEET ALERT
🧬 Genetic Engineering Guide!
🔬 Key Concepts in Genetic Engineering
1) CRISPR-Cas9 gene editing mechanism
2) Plasmid vector design principles
3) Gene expression regulation systems
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